Anti-Tissue Transglutaminase ELISA Test Kit - India CDSCO Medical Device Registration

Product Information

Anti Tissue Transglutaminase is an indirect solid phase enzyme immunometric assay (NA) kit designed for the quantitative measurement of IgA class antibodies directed against tissue transglutaminase in human serum or plasma. The assay is intended for in vitro diagnostic use only as an aid in the diagnosis of celiac disease and dermatitis herpetiformis. Anti Tissue Transglutaminase IgA test is based on the binding of serum or plasma antibodies on the human recombinant tissue transglutaminase coated into the microplates. The antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human-IgA horseradish peroxidase conjugate solution recognize IgA class antibodies bound to the immobilized antigens. After 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solutions color change into yellow. The amount of color is directly proportional to the concentration of IgA antibodies present in the original sample